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p smad 1 5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p smad 1 5
    P Smad 1 5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+smad+1+5/pm41898356-69-7-9
    Average 86 stars, based on 1 article reviews
    p smad 1 5 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Genetic or therapeutic neutralization of ALK1 reduces LDL transcytosis and atherosclerosis in mice.
    Article Snippet: A total of 20 μg of protein was loaded into NuPAGE 3–8% Tri Acetate Gel (Invitrogen): 10% sodium dodecylsulfate–polyacrylamide gel electrophoresis gel followed by transfer to nitrocellulose membranes. .. Western blotting was performed with the following primary antibodies: apoB (Meridian Life Science, Inc., catalog no. K23300R), HSP90 (BD Biosciences, catalog no. 610419), ALK1 (Fitzgerald, catalog no. 70R-49334) and p-SMAD 1/5 (Cell Signaling, catalog no. 9516). ..

    Ubiquitin Proteomics:

    Article Title: Estrogen and Cyp1b1 Regulate Pparγ in Pulmonary Hypertension Through a Ubiquitin-Dependent Mechanism.
    Article Snippet: CD36 and PGC1α antibodies were purchased from NOVUS (NOVUS biologicals, USA). .. PPARγ was purchased from Abcam (Abcam 45036, USA). p‐smad 1/5 (13820S), p‐smad 2/3 (8828S), p‐AKT (4060S), p‐p44/42 (4376S), p‐p38 (9211S), ubiquitin (3936S) were purchased from Cell Signaling Technology (CST, catalog numbers given above, USA). ..

    Article Title: Estrogen and Cyp1b1 Regulate Pparγ in Pulmonary Hypertension Through a Ubiquitin‐Dependent Mechanism
    Article Snippet: CD36 and PGC1α antibodies were purchased from NOVUS (NOVUS biologicals, USA). .. PPARγ was purchased from Abcam (Abcam 45036, USA). p‐smad 1/5 (13820S), p‐smad 2/3 (8828S), p‐AKT (4060S), p‐p44/42 (4376S), p‐p38 (9211S), ubiquitin (3936S) were purchased from Cell Signaling Technology (CST, catalog numbers given above, USA). ..

    Incubation:

    Article Title: Repeated administration of adipose-derived mesenchymal stem cells added on beneficial effects of empagliflozin on protecting renal function in diabetic kidney disease rat
    Article Snippet: .. The membranes were incubated with the indicated primary antibodies to detect the parameters' amount [matrix metalloproteinase 9 (MMP9) (1: 1000, Abcam), phosphorylated nuclear factor p–NF)–kB (1: 1000, Cell Signaling), NOX-1 (1: 1000, Sigma), NOX-2 (1: 1000, Sigma), cleaved poly ADP ribose polymerase (PARP) (1: 1000, Cell Signaling), mitochondrial Bax (1: 1000, Abcam), tumor necrosis factor (TNF)-α (1: 1000, Cell Signaling), P22 phox (1: 1000, Abcam), p-Smad 3 (1: 1000, Cell Signaling), transforming growth factor (TGF)-β (1:1000, Abcam), p-Smad 1/5 (1: 1000, Cell Signaling), bone morphogenetic protein (BMP)2 (1: 1000, Abcam), phosphorylated dynamin-related protein 1DRP1 (1: 1000, Cell Signaling), γ-H2AX (1: 1000, Cell Signaling), Atg 5 (1: 1000, Cell Signaling), cleaved caspase 3 (1: 1000, Cell Signaling), oxidized protein (1:100, Millipore) and Actin (1: 10,000, Chemicon)] for 1 h at room temperature. .. Horseradish peroxidase-conjugated secondary antibodies were further incubated for 1 h at room temperature and performed by enhanced chemiluminescence (ECL; Amersham Biosciences, Amersham, UK), followed by exposure to Biomax L film (Kodak, Rochester, NY, USA).

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    Article Title: Sclerostin inhibits Protein kinase C inhibitor GÖ6976 induced osteogenic differentiation of dental follicle cells.
    Article Snippet: Human dental follicle cells (DFCs) as multipotent stem cells are currently investigated within the field of regenerative medicine considering their potential for the regeneration of dental tissues, bone defects caused by periodontal or degenerative diseases and the treatment of craniofacial disorders.. However, molecular mechanisms of the differentiation into mineralizing cells are still inadequately understood.. Previous studies have shown that GÖ6976, an inhibitor of classical isoforms of protein kinase C (PKC), enhanced ostogenic differentiation of DFCs.

    Lysis:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    SDS Page:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    Saline:

    Article Title: Supporting Information for Fibrillin-1 regulates endothelial sprouting during angiogenesis
    Article Snippet: .. Lysis was performed directly in Laemmli sample buffer and samples were run on a 10% SDS-PAGE gel at 120 V for 90 min. Proteins were transferred onto Immobilon polyvinylidene difluoride membranes (Merck Millipore) at 120 V for 1 h. Membranes were saturated with 5% bovine serum albumin in Tris-buffered saline containing 0.1 % Tween 20 for 1h and incubated overnight at 4°C with primary antibodies against fibronectin (Abcam, #ab6328), Dll4 (Cell Signaling Technologies (CST) #2589), Jagged-1 (CST #70109), Smad1 (CST #9743), Smad6 (Novus, #NB100-56440), MAGP1 (Santa Cruz, #sc-166075), PVEGFR2 (CST, #2478), P-VEGFR3 (Cell application, # CY1115) P-ERK1/2 (CST #4370), P-JNK (CST #4668), P-eNOS (CST #9571), P-Smad 1/5 (CST, #9516), P-AKT (CST #9271), NRP1 (R&D Systems, #AF566), VEGFR2 (R&D Systems, #AF357), ADAMTS1 (R&D Systems, #AF5867), VEGFR3 (R&D Systems, #AF349), NICD (CST #4147), NTM (CST #3608), P-Smad2/3 (Sigma #04-953), -actinin (CST #6487), -tubulin (Sigma #T9026). .. Then, primary antibodies were revealed using horseradish peroxidase-coupled anti-mouse, anti-goat, anti-sheep or anti-rabbit (Jackson ImmunoResearch) secondary antibodies for 1 h. Finally, horseradish peroxidase activity was revealed using a chemiluminescence kit (GE-healthcare) according to the manufacturer’s instructions.

    Membrane:

    Article Title: Sclerostin inhibits Protein kinase C inhibitor GÖ6976 induced osteogenic differentiation of dental follicle cells.
    Article Snippet: Human dental follicle cells (DFCs) as multipotent stem cells are currently investigated within the field of regenerative medicine considering their potential for the regeneration of dental tissues, bone defects caused by periodontal or degenerative diseases and the treatment of craniofacial disorders.. However, molecular mechanisms of the differentiation into mineralizing cells are still inadequately understood.. Previous studies have shown that GÖ6976, an inhibitor of classical isoforms of protein kinase C (PKC), enhanced ostogenic differentiation of DFCs.



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